Vasudev earned a bachelor's degree in English from the University of Mysore and has been teaching yoga in southern India since 1982. In 1992, he established Isha Foundation near Coimbatore, which runs an ashram, hosts a yoga centre, and has been involved in various activities in spirituality, education, and the environment.

Vasudev is the author of several books, including Inner Engineering: A Yogi's Guide to Joy (2016). As a public speaker he has spoken at the United Nation's Millennium World Peace Summit, the British parliament's House of Lords, the Massachusetts Institute of Technology, and the International Institute for Management Development. He has also spoken at the annual World Economic Forum in 2007, 2017 and 2020.

In 2017, he was awarded the Padma Vibhushan, India's second highest civilian award, by the Government of India for his services in social services.

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