Despicable Me Minion Mayhem is a computer-animated simulator ride attraction located at Universal Studios Florida, Universal Studios Hollywood, and Universal Studios Japan and upcoming to Universal Studios Singapore and Universal Studios Beijing. The attraction is based on Universal Pictures and Illumination's animated film Despicable Me (2010) and its franchise, employing the use of 3D high-definition digital animation. The attraction replaced Jimmy Neutron's Nicktoon Blast in Florida, T2-3D: Battle Across Time in Hollywood, Back to the Future: The Ride in Japan and will replace Madagascar: A Crate Adventure in Singapore. It will also be an opening day attraction in Beijing.

Universal Orlando Resort announced that the attraction is being designed by their Universal Creative company in association with Illumination Entertainment and Despicable Me film producer Chris Meledandri. The ride is a motion simulator and features multiple characters from the film, including Gru, Margo, Edith, Agnes, and the Minions.

The attraction at Universal Studios Florida is located in Soundstage 42 in the Production Central area. The soundstage previously housed The Funtastic World of Hanna-Barbera from 1990 until 2002 and Jimmy Neutron's Nicktoon Blast from 2003 until 2011. The attraction at Universal Studios Hollywood is located on the former site of Terminator 2 3D: Battle Across Time on the Upper Lot (Entertainment Center). The attraction at Universal Studios Japan is located on the former site of Back to the Future: The Ride in the San Francisco area in a new area called Minion Park. The ride is also slated to open in 2021 at Universal Studios Singapore.

After the ride (in Florida and Hollywood), the doors open to a dance party with the public dancing to "Boogie Fever," along with a costumed Minion. Also, the exit corridors are fitted with rainbow lights and 'Fun, Fun, Fun' plays as the guests exit down some ramps.

In May 2012, the ride's gift shop Super Silly Stuff opened at Universal Studios Florida, replacing the Nickstuff store. The Hollywood version opened in March 2014 replacing T2 Gear & Supply Co. The store sells Despicable Me-related merchandise. The store is themed to the theme park Gru, Margo, Edith, and Agnes visited in the first Despicable Me film, Super Silly Fun Land. At Universal Studios Japan, an alternate version called the Minion Mart opened in mid-2015, replacing the Back to the Future Gifts store. However, this was closed and replaced by multiple other shops with the opening of Minion Park and the ride.

Voice cast
Steve Carell - Gru
Miranda Cosgrove - Margo
Dana Gaier - Edith
Elsie Fisher - Agnes
Pierre Coffin - the Minions

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