Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use.

Table of Contents:
00:00 Intro
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
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Factual References (and excellent for learning more detail about this topic):

Clark, Mary Ann, et al. “17.1 Biotechnology - Biology 2e | OpenStax.” Biology 2e, OpenStax, 28 Mar. 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology

https://www.fda.gov/consumers/consumer-updates/coronavirus-testing-basics

https://www.fda.gov/media/136151/download

*General length of a primer in base pairs from NIH https://www.genome.gov/genetics-glossary/Primer
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FURTHER READING SUGGESTIONS:

On RT-PCR for SARS-CoV-2:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197457/
https://jamanetwork.com/journals/jama/fullarticle/2765837

On Fluorescent Probes in PCR:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC52277/?page=1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148886/

What about limitations or disadvantages of PCR?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102308/

What about more uses for PCR (as well as more info about Nobel Prize):
https://www.nobelprize.org/prizes/chemistry/1993/press-release/
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Turkish subtitles by Kardelen Ünalleylioğlu
Hindi subtitles by Alisha Aggarwal

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