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Elisa Test | Direct Indirect & Sandwich 🩸

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▬▬▬▬▬▬▬▬▬▬ Contents of this video ▬▬▬▬▬▬▬▬▬▬

00:00:00 What Is ELISA?
00:00:40 Explanation Of Mechanism Through An Example
00:09:57 Addition Of Substrate
00:11:55 Advantages Of Direct ELISA Test
00:13:58 Issues And Modifications Of ELISA
00:19:50 What Is Epitope?
00:22:01 Indirect Elisa Test And Its Mechanism
00:26:50 Performance Of Direct And Indirect Elisa Test
00:33:48 Effect Of Adding More Enzymes
00:34:40 When To Use Direct Or Indirect Test
00:39:44 Purpose Of Using These Two Tests Together
00:42:32 Sandwich Modification
00:49:37 Difference Between Direct And Direct Sandwich Elisa
00:51:03 Indirect Sandwich Test
00:54:27 Rapid Summary
00:58:07 Question
01:01:25 Radioimmuno Assay And Its Comparison With ELISA

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The ELISA test is simple and straightforward. You’ll probably need to sign a consent form, and your doctor should explain the reason for doing the test.

The ELISA test involves taking a sample of your blood. First, a healthcare provider will cleanse your arm with an antiseptic. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. Next, a needle will be placed in one of your veins to draw a small sample of blood. When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. You’ll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow.

This procedure should be relatively painless, but your arm may throb a little after it’s done.

The blood sample will be sent to a laboratory for analysis. In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. If your blood contains antibodies to the antigen, the two will bind together. The technician will check this by adding an enzyme to the petri dish and observing how your blood and the antigen react.

You may have the condition if the contents of the dish change color. How much change the enzyme causes allows the technician to determine the presence and amount of antibody
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